6PI9
Crystal structure of 16S rRNA methyltransferase RmtF in complex with S-Adenosyl-L-homocysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 77.637, 77.637, 96.926 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.829 - 1.850 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3frh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15_3448: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.059 | 1.626 |
| Rpim | 0.017 | 0.489 |
| Number of reflections | 26247 | 1291 |
| <I/σ(I)> | 41.08 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 13.8 | 11.5 |
| CC(1/2) | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 298 | 3.5 M sodium formate, 0.1 M sodium acetate, pH 4.8, 2 mM S-adenosyl-L-methionine |
