6PI8
Crystal structure of Marinobacter subterrani acetylpolyamine amidohydrolase (msAPAH) complexed with acetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.371, 121.060, 66.556 |
| Unit cell angles | 90.00, 109.53, 90.00 |
Refinement procedure
| Resolution | 28.081 - 1.635 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.19350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB: 4ZUM |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.853 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.081 | 1.690 |
| High resolution limit [Å] | 1.635 | 1.640 |
| Rpim | 0.066 | 0.531 |
| Number of reflections | 95962 | 9560 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10 mg/ml msAPAH Protein, 0.2 M calcium acetate hydrate, 20% w/v PEG 3350, 1:1 ratio protein to precipitant |
