6PI6
The evolving story of AtzT, a periplasmic binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953716 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.761, 62.786, 120.173 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 49.100 - 1.650 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pii |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.604 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.086 | 1.265 |
| Rpim | 0.035 | 0.515 |
| Number of reflections | 176707 | 8668 |
| <I/σ(I)> | 10.8 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.998 | 0.653 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein at 20 mg/mL in SD2 plates incubated at 20 C. Crystallisation conditions contained 6-8% Jeffamine M600 with 2-7% MPD and trisodium citrate at 1.1-1.2 M |
