6PH7
Crystal structure of bovine opsin with nerol bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 243.137, 243.137, 108.427 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.256 - 2.902 |
| R-factor | 0.2145 |
| Rwork | 0.214 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.662 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.730 | 2.912 |
| High resolution limit [Å] | 2.900 | 2.902 |
| Rmerge | 0.054 | 0.588 |
| Rmeas | 0.060 | |
| Rpim | 0.024 | 0.330 |
| Number of reflections | 26899 | 1749 |
| <I/σ(I)> | 20.6 | 2.9 |
| Completeness [%] | 99.1 | 97.8 |
| Redundancy | 5.9 | 6.6 |
| CC(1/2) | 0.999 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 3.1-3.3 ammonium sulfate, 0.1M sodium acetate buffer, pH 5.5 |
