6PH7

Crystal structure of bovine opsin with nerol bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	173
Detector technology	PIXEL
Collection date	2016-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	H 3 2
Unit cell lengths	243.137, 243.137, 108.427
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.256 - 2.902
R-factor	0.2145
Rwork	0.214
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.662
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.730	2.912
High resolution limit [Å]	2.900	2.902
Rmerge	0.054	0.588
Rmeas	0.060
Rpim	0.024	0.330
Number of reflections	26899	1749
<I/σ(I)>	20.6	2.9
Completeness [%]	99.1	97.8
Redundancy	5.9	6.6
CC(1/2)	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	3.1-3.3 ammonium sulfate, 0.1M sodium acetate buffer, pH 5.5