6PH4

Full length LOV-PAS-HK construct from the LOV-HK sensory protein from Brucella abortus (light-adapted, construct 15-489)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	95.956, 104.662, 164.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.520 - 3.250
R-factor	0.2581
Rwork	0.255
R-free	0.31780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3T50 and 5EPV
RMSD bond length	0.003
RMSD bond angle	0.686
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.530	3.470
High resolution limit [Å]	3.250	3.250
Rmeas	0.118	2.774
Number of reflections	26861	4778
<I/σ(I)>	13.1	1
Completeness [%]	100.0	100
Redundancy	14.8	15.3
CC(1/2)	0.998	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	294	15% (v/v) MPD + 4% (w/v) PEG 4000 + 0.1 M Hepes, pH 7.2