6PH2
Complete LOV domain from the LOV-HK sensory protein from Brucella abortus (mutant C69S, construct 15-155)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.270, 95.860, 107.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.630 - 2.340 |
R-factor | 0.22909 |
Rwork | 0.227 |
R-free | 0.26071 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t50 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.274 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.630 | 2.490 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmeas | 0.154 | 0.624 |
Number of reflections | 29308 | 4523 |
<I/σ(I)> | 11.6 | 3.3 |
Completeness [%] | 99.4 | 96.7 |
Redundancy | 7.1 | 7.1 |
CC(1/2) | 0.997 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 294 | 15% (w/v) PEG 3350 + 0.1 M sodium citrate, pH 5.2 |