6PGZ
MTSL labelled T4 lysozyme pseudo-wild type V75C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.941, 53.328, 58.607 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 14.955 - 2.000 |
| R-factor | 0.255 |
| Rwork | 0.253 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vnr |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.074 | 0.048 | 0.277 |
| Number of reflections | 19008 | 2093 | 2140 |
| <I/σ(I)> | 12.2 | 3.3 | |
| Completeness [%] | 89.1 | 95.1 | 100 |
| Redundancy | 2.8 | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.0M Na/K Phosphate, 250mM NaCl, pH7.0 |
