6PGS
Crystal structure of bovine opsin with geraniol bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2015-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 243.919, 243.919, 109.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.206 - 2.905 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.23540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j4q |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.552 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.300 | 3.070 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.079 | 1.019 |
| Rmeas | 0.086 | 1.142 |
| Rpim | 0.034 | 0.509 |
| Number of reflections | 27357 | 4495 |
| <I/σ(I)> | 16.7 | 2.2 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 6.1 | 9.7 |
| CC(1/2) | 0.999 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 3.1-3.3M ammonium sulfate, 0.1M sodium acetate buffer, pH 5.5 |
