6PGN

PagF single mutant with GPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-26
Detector	RAYONIX MX300HE
Wavelength(s)	0.978560
Spacegroup name	P 65
Unit cell lengths	116.662, 116.662, 43.681
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.850
R-factor	0.19034
Rwork	0.189
R-free	0.21556
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tty
RMSD bond length	0.008
RMSD bond angle	1.272
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	SHARP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.600	1.856
High resolution limit [Å]	1.850	1.850
Number of reflections	29288
<I/σ(I)>	24.6
Completeness [%]	100.0
Redundancy	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		282	lithium sulfate, PEG3350