6PGM
PirF geranyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-14 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.978560 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.898, 93.911, 93.831 |
| Unit cell angles | 90.00, 128.51, 90.00 |
Refinement procedure
| Resolution | 48.082 - 2.300 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tty |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | pointless |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 2.320 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 37152 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 98.2 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | Bis-tris propane, sodium citrate, ammonium sulfate |
