6PGK
Membrane Protein Megahertz Crystallography at the European XFEL, Photosystem I XFEL at 2.9 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | EUROPEAN XFEL BEAMLINE SPB/SFX |
| Synchrotron site | European XFEL |
| Beamline | SPB/SFX |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2017-11-24 |
| Detector | AGIPD |
| Wavelength(s) | 1.399 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 279.100, 164.600, 284.100 |
| Unit cell angles | 90.00, 119.25, 90.00 |
Refinement procedure
| Resolution | 39.880 - 2.900 |
| R-factor | 0.3 |
| Rwork | 0.298 |
| R-free | 0.33596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jb0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.210 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.880 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 493312 | 49038 |
| <I/σ(I)> | 3.5 | 0.47 |
| Completeness [%] | 99.4 | 93.9 |
| Redundancy | 94 | 52 |
| CC(1/2) | 0.920 | 0.023 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.4 | 283 | 0-3 mM MgSO4 5 mM MES 0.02 % beta-DDM |
