6PGB
WDR5delta32 bound to N-(4-(5-(hydroxymethyl)-1H-imidazol-2-yl)butyl)-2-phenylacetamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.798, 65.275, 97.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.560 - 1.730 |
R-factor | 0.17612 |
Rwork | 0.174 |
R-free | 0.21697 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6pg4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.407 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.560 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Number of reflections | 31184 | 1348 |
<I/σ(I)> | 12.6 | 2.8 |
Completeness [%] | 98.1 | 80 |
Redundancy | 13.6 | |
CC(1/2) | 0.996 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.101 M ammonium sulfate, 0.1 M Bis-Tris chloride, pH 5.63, 25.8% w/v PEG4000 |