6PG6
WDR5delta23 bound to N-(4-(5-(hydroxymethyl)-1H-imidazol-2-yl)butyl)acetamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 112.366, 46.974, 129.274 |
| Unit cell angles | 90.00, 112.75, 90.00 |
Refinement procedure
| Resolution | 43.740 - 1.680 |
| R-factor | 0.16684 |
| Rwork | 0.165 |
| R-free | 0.19337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pg3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.740 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 70355 | 3377 |
| <I/σ(I)> | 17.5 | 2.7 |
| Completeness [%] | 99.0 | 94.6 |
| Redundancy | 7.4 | 7.1 |
| CC(1/2) | 0.999 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.215 M ammonium sulfate, 0.1 M sodium cacodylate (pH 7.06), 25.6 %w/v PEG8000 |
