6PG0

Protein Tyrosine Phosphatase 1B (1-301), P188A mutant, vanadate bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-23
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	88.227, 88.227, 104.068
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.800 - 2.100
Rwork	0.195
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qkq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.800	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.062
Rmeas	0.067
Number of reflections	26477	15601
<I/σ(I)>	17.74
Completeness [%]	95.0
Redundancy	6.8
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1M Hepes, 0.2 M magnesium acetate and 15-20% polyethylene glycol 8000