6PFS

rsEGFP2 with a chlorinated chromophore in the fluorescent on-state in a contracted unit cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	51.422, 59.246, 65.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.711 - 1.759
R-factor	0.1809
Rwork	0.178
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtx
RMSD bond length	0.004
RMSD bond angle	0.762
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.711	1.822
High resolution limit [Å]	1.759	1.759
Rmerge	0.038	0.446
Rmeas	0.054	0.631
Number of reflections	20330	1968
<I/σ(I)>	8.52	1.68
Completeness [%]	99.6	98.35
Redundancy	2	2
CC(1/2)	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	298	1.76 M ammonium sulfate, 0.1 M HEPES; Cryoprotectant 1.6 M sucrose, 2.72 M ammonium sulfate, 0.16 M HEPES.