6PFP

Crystal Structure of Amino Acids 1473-1536 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-08
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97915
Spacegroup name	P 1
Unit cell lengths	37.107, 59.304, 86.548
Unit cell angles	81.79, 86.42, 89.97

Refinement procedure

Resolution	37.177 - 2.200
R-factor	0.1962
Rwork	0.194
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INOH
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.092	0.062	0.306
Rmeas	0.106	0.073	0.353
Rpim	0.050	0.038	0.172
Total number of observations	128524
Number of reflections	35454	1807	1499
<I/σ(I)>	7.1
Completeness [%]	95.5	97.7	83.8
Redundancy	3.6	4	2.7
CC(1/2)		0.988	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Crystals grew from 1:1 mixture of 18 mg/ml protein solution with 37% (w/v) 2-methyl-2,4-pentanediol (MPD), 80 mM magnesium sulfate, 100 mM sodium acetate. Protein solution contained 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) pH 7.6, and 0.2 mM tris(2-carboxyethyl)phosphine (TCEP).