Experimental procedure
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.000, 115.200, 96.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.25500 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.039 |
| Data reduction software | MOSFLM |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.126 |
| Number of reflections | 44349 |
| Completeness [%] | 97.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.8 | THE CRYSTALS WERE GROWN FROM 1.6 - 1.8M POTASSIUM TARTRATE, PH 7.8, WITH 10MM 2-PHOSPHOGLYCOLATE, 0.5MM EDTA, 0.5MM DTT. THE CRYSTALS WERE GROWN FROM 1.6 - 1.8M POTASSIUM TARTRATE, PH 7.8, WITH 10MM 2-PHOSPHOGLYCOLATE, 0.5MM EDTA, 0.5MM DTT. 2-PHOSPHOGLYCOLATE IS AN ANALOGUE OF THE NATURAL ALLOSTERIC INHIBITOR PHOSPHOENOLPYRUVATE, SO THIS STRUCTURE REPRESENTS THE ALLOSTERICALLY INHIBITED T-STATE. THE ASYMMETRIC UNIT OF THE CRYSTAL IS THE TETRAMER WHICH OCCURS IN SOLUTION - CHAINS A, B, C, D. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | potassium tartrate | 1.6-1.8 (M) | |
| 2 | 1 | 1 | PGC | 10 (mM) | |
| 3 | 1 | 1 | EDTA | 0.5 (mM) | |
| 4 | 1 | 1 | DTT | 0.5 (mM) | |
| 5 | 1 | 1 | protain | 9-6 (mg/ml) |
