6PF2

Crystal Structure of Amino Acids 1220-1276 of Human Beta Cardiac Myosin Fused to Gp7 and Eb1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-15
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97904
Spacegroup name	P 21 21 21
Unit cell lengths	37.136, 44.607, 262.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.976 - 2.170
R-factor	0.206
Rwork	0.204
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1noh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.210
High resolution limit [Å]	2.170	5.890	2.170
Rmerge	0.095	0.049	0.519
Rmeas	0.100	0.051	0.559
Rpim	0.030	0.016	0.201
Total number of observations	232416
Number of reflections	22944	1394	809
<I/σ(I)>	6.9
Completeness [%]	94.0	99.5	68.8
Redundancy	10.1	11.1	5.6
CC(1/2)		1.000	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Crystals grew spontaneously at room temperature by mixing 1:1 ratio of 16 mg/ml protein in 100 mM NaCl, 10 mM HEPES pH 7.6, 0.1 mM TCEP with well solution containing 2.4 M ammonium phosphate pH 8.0, 100 mM HEPPS pH 8.0.