6PD4
Crystal Structure of Hendra Virus Attachment G Glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.152, 73.244, 109.132 |
Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
Resolution | 36.924 - 2.200 |
R-factor | 0.1687 |
Rwork | 0.167 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.079 | 0.041 | 0.237 |
Total number of observations | 169409 | ||
Number of reflections | 51943 | 5456 | 4697 |
<I/σ(I)> | 7.9 | ||
Completeness [%] | 97.4 | 99.8 | 88.6 |
Redundancy | 3.3 | 3.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG2000MME and 130 mM (NH4)2SO4 |