6PCZ
Crystal structure of the bacterial cellulose synthase subunit G (BcsG) catalytic domain from Escherichia coli, selenomethionine variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-07 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97965 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.710, 94.580, 105.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.970 - 1.440 |
| R-factor | 0.1969 |
| Rwork | 0.196 |
| R-free | 0.20830 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 1.491 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.062 | 1.051 |
| Rmeas | 0.068 | 1.140 |
| Rpim | 0.026 | 0.440 |
| Number of reflections | 142124 | 14051 |
| <I/σ(I)> | 19.13 | 1.74 |
| Completeness [%] | 100.0 | 99.99 |
| Redundancy | 6.5 | 6.7 |
| CC(1/2) | 0.999 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES:NaOH, 20% (w/v) PEG 4000, 10% (v/v) 2-propanol |
