6PBQ
Structure of ClpC1-NTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.663, 63.403, 36.752 |
| Unit cell angles | 90.00, 115.53, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.600 |
| R-factor | 0.17887 |
| Rwork | 0.177 |
| R-free | 0.21287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cn8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.943 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.062 | 0.117 |
| Rpim | 0.033 | 0.061 |
| Number of reflections | 18428 | 899 |
| <I/σ(I)> | 21 | |
| Completeness [%] | 94.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1 M HEPES, pH 6.5, 20% PEG6000 |
