6PBN
Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 1 hour
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.023, 53.842, 96.867 |
Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
Resolution | 39.960 - 1.650 |
R-factor | 0.1743 |
Rwork | 0.174 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6c4n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.238 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.960 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 110062 | 4833 |
<I/σ(I)> | 12.9 | 2 |
Completeness [%] | 98.5 | |
Redundancy | 6.8 | |
CC(1/2) | 0.998 | 0.727 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 260 mM ammonium formate, and 25% PEG 3350 |