6PBM
Pseudopaline Dehydrogenase with NADP+ bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.854, 53.865, 96.881 |
| Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
| Resolution | 39.640 - 1.570 |
| R-factor | 0.1652 |
| Rwork | 0.165 |
| R-free | 0.18180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c4n |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.640 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 126498 | 5877 |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 98.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.997 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.45 | 298 | 100 mM BisTris pH 5.45, 200 mM ammonium acetate, and 24% PEG 3350 |
