6PBG

Crystal structure of WD-repeat domain of human coatomer subunit Alpha (COPA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-12
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	34.633, 85.452, 47.913
Unit cell angles	90.00, 107.04, 90.00

Refinement procedure

Resolution	20.760 - 1.720
R-factor	0.1727
Rwork	0.171
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j87
RMSD bond length	0.008
RMSD bond angle	1.352
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.750
High resolution limit [Å]	1.720	4.670	1.720
Rmerge	0.088	0.043	0.472
Rmeas	0.096	0.047	0.532
Rpim	0.037	0.018	0.236
Total number of observations	170533
Number of reflections	26521	1433	977
<I/σ(I)>	8.3
Completeness [%]	93.6	97.9	69.3
Redundancy	6.4	6.6	4.1
CC(1/2)		0.999	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M di-Ammonium Tartrate