6PAR

Structure of a bacterial Atm1-family ABC exporter with MgAMPPNP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	169.648, 92.498, 237.691
Unit cell angles	90.00, 110.34, 90.00

Refinement procedure

Resolution	39.619 - 3.350
R-factor	0.2551
Rwork	0.254
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pan
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.619	3.670	3.470
High resolution limit [Å]	3.350	3.600	3.350
Rmerge	0.174	1.942	1.768
Rmeas	0.189	2.100	1.927
Rpim	0.071	0.787	0.754
Number of reflections	98507	4376	9041
<I/σ(I)>	9.43	1.1	0.97
Completeness [%]	97.8	95.8	91.11
Redundancy	7	6.9	6.4
CC(1/2)	0.999	0.466	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	296	ADA, PEG1500, PEG1000
1	VAPOR DIFFUSION, HANGING DROP	7.1	296	ADA, PEG1500, PEG1000