6PAQ

Structure of a bacterial Atm1-family ABC exporter with ATP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	89.346, 115.354, 184.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.626 - 3.301
R-factor	0.2374
Rwork	0.235
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pan
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.630	3.419
High resolution limit [Å]	3.300	3.300
Rmerge	0.190	2.680
Rmeas	0.200	2.784
Rpim	0.054	0.748
Number of reflections	29277	2861
<I/σ(I)>	11.11	1.01
Completeness [%]	99.3	98.72
Redundancy	13.2	13.6
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	296	NaCl, Tris, PEG2000MME