6PA8
ECAII(T89V,K162T) MUTANT IN COMPLEX WITH L-ASN AT PH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-12 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.793, 62.746, 140.966 |
| Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.1489 |
| Rwork | 0.146 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.150 | 1.900 |
| Rmerge | 0.044 | 0.016 | 0.425 |
| Rmeas | 0.053 | 0.019 | 0.548 |
| Rpim | 0.029 | 0.010 | 0.341 |
| Total number of observations | 262551 | ||
| Number of reflections | 88385 | 4768 | 3598 |
| <I/σ(I)> | 13.6 | ||
| Completeness [%] | 95.3 | 99.3 | 78.5 |
| Redundancy | 3 | 3.5 | 2 |
| CC(1/2) | 0.999 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Crystals were grown from 0.17 M NH4-citrate, pH 7.0, 17-18% PEG3350, then soaked in equivalent solution containing L-Asn at concentration 0.5 mM |
