6PA0

Structure of the G77A mutant in Sodium Chloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	I 4
Unit cell lengths	155.018, 155.018, 76.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.660 - 2.050
R-factor	0.1968
Rwork	0.196
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.854
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.660	34.660
High resolution limit [Å]	2.050	2.050
Number of reflections	55579	55579
<I/σ(I)>	15.76
Completeness [%]	83.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	22-26 % PEG400 (v/v), 50 mM magnesium acetate, 50 mM sodium acetate (pH 4.8.-5.4)