6P93
Human APE1 K98A AP-endonuclease product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-27 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.351, 60.493, 72.620 |
| Unit cell angles | 83.72, 79.66, 88.46 |
Refinement procedure
| Resolution | 24.966 - 2.100 |
| R-factor | 0.1745 |
| Rwork | 0.172 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.140 |
| High resolution limit [Å] | 2.087 | 5.680 | 2.100 |
| Rmerge | 0.073 | 0.035 | 0.426 |
| Rmeas | 0.084 | 0.040 | 0.551 |
| Rpim | 0.039 | 0.018 | 0.344 |
| Number of reflections | 58444 | 2178 | 2143 |
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.8 | 100 | 98.2 |
| Redundancy | 3.9 | 4.4 | 2.1 |
| CC(1/2) | 0.998 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% PEG20000, 100 mM sodium citrate, pH 5.0, and 200 mM MgCl2 |
