6P8V

Structure of E. coli MS115-1 HORMA:CdnC:Trip13 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	89.789, 138.516, 100.212
Unit cell angles	90.00, 98.18, 90.00

Refinement procedure

Resolution	88.876 - 2.640
R-factor	0.235
Rwork	0.233
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p80
RMSD bond length	0.004
RMSD bond angle	0.662
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.190	2.700
High resolution limit [Å]	2.640	2.640
Rmerge	0.142	1.186
Rmeas	0.170	1.426
Rpim	0.091	0.783
Number of reflections	70640	4550
<I/σ(I)>	7.2	1.1
Completeness [%]	99.2	99.6
Redundancy	3.4	3.2
CC(1/2)	0.992	0.292

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M MOPS pH 7.0, 0.2 M MgCl2, and 14% PEG 3350