6P8U

Structure of P. aeruginosa ATCC27853 CdnD:HORMA2:Peptide 1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	169.087, 43.373, 63.217
Unit cell angles	90.00, 104.18, 90.00

Refinement procedure

Resolution	81.966 - 1.893
R-factor	0.1907
Rwork	0.189
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p82 6p8r
RMSD bond length	0.005
RMSD bond angle	0.586
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.970	1.940
High resolution limit [Å]	1.890	1.890
Rmerge	0.134	1.302
Rmeas	0.159	1.554
Rpim	0.086	0.837
Number of reflections	34946	1989
<I/σ(I)>	7.1	0.8
Completeness [%]	97.7	87
Redundancy	3.3	3.2
CC(1/2)	0.990	0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	100 mM Bis-Tris pH 5.5, 0.2 M ammonium acetate, and 30% PEG 3350