6P8S

Structure of P. aeruginosa ATCC27853 HORMA1:HORMA2:Peptide 1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	100.892, 124.539, 109.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.197 - 2.000
R-factor	0.1931
Rwork	0.191
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p8p 6p8r
RMSD bond length	0.006
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.200	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.219	1.878
Rmeas	0.232	2.043
Rpim	0.074	0.787
Number of reflections	44741	2481
<I/σ(I)>	8.3	1
Completeness [%]	96.0	73.7
Redundancy	9.6	6.5
CC(1/2)	0.995	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM HEPES pH 7.5, 0.5 M Sodium acetate, 31% PEG 3350, and 1 mM ATP