6P8R

Structure of P. aeruginosa ATCC27853 HORMA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 41
Unit cell lengths	106.106, 106.106, 74.789
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.130 - 2.141
R-factor	0.2041
Rwork	0.203
R-free	0.23260
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.566
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.110	2.200
High resolution limit [Å]	2.140	2.140
Rmerge	0.064	1.402
Rmeas	0.070	1.524
Rpim	0.028	0.588
Number of reflections	45579	3448
<I/σ(I)>	22.6	1.2
Completeness [%]	99.3	91.5
Redundancy	6.6	6.5
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	100 mM Imidazole pH 8.0, 0.8 M NaH2PO4, and 0.55-0.8 M KH2PO4