6P8O

Structure of P. aeruginosa ATCC27853 HORMA2-deltaC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	65.421, 30.585, 74.911
Unit cell angles	90.00, 101.57, 90.00

Refinement procedure

Resolution	44.094 - 1.604
R-factor	0.1919
Rwork	0.191
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p8r
RMSD bond length	0.005
RMSD bond angle	0.712
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.062	0.474
Rmeas	0.074	0.581
Rpim	0.040	0.330
Number of reflections	38122	1759
<I/σ(I)>	18.4	1.4
Completeness [%]	98.5	92.2
Redundancy	3.1	2.7
CC(1/2)	0.992	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris-HCl pH 8.5, 10 mM NiCl2, and 20% PEG 2000 MME.