6P8N

Crystal Structure of Antibody P-p1f1 in Complex with eOD-GT8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	60.879, 92.406, 223.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.165 - 3.202
R-factor	0.2291
Rwork	0.226
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jpk
RMSD bond length	0.003
RMSD bond angle	0.665
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	20701	1703
<I/σ(I)>	7.89	1.58
Completeness [%]	98.7	99.7
Redundancy	6.2	5.8
CC(1/2)	0.970	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	22.5% PEG 3350, 13.5% Isopropanol, 0.18M Ammonium Citrate pH 4.0