6P8I
N-terminal 5 domains of IGFIIR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.788, 66.228, 123.070 |
| Unit cell angles | 90.00, 99.18, 90.00 |
Refinement procedure
| Resolution | 39.970 - 2.540 |
| R-factor | 0.2285 |
| Rwork | 0.224 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q25 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.106 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.590 |
| High resolution limit [Å] | 2.540 | 6.910 | 2.550 |
| Rmerge | 0.068 | 0.026 | 0.649 |
| Number of reflections | 48916 | 1385 | 1270 |
| <I/σ(I)> | 16.5 | ||
| Completeness [%] | 99.3 | 98.9 | 98 |
| Redundancy | 3.2 | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292.15 | 100mM ammonium citrate dibasic, 20% PEG 3350 |
