6P8G
Crystal structure of CDK4 in complex with CyclinD1 and P27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 62.580, 66.730, 184.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.730 - 2.800 |
| R-factor | 0.2239 |
| Rwork | 0.221 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.520 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.420 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.190 | 0.820 |
| Rpim | 0.070 | 0.310 |
| Number of reflections | 19805 | 2851 |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.1 | 7.5 |
| CC(1/2) | 0.980 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 100mM Tris (7.0) 200mM MgCl2 10% PEG 8000 |
