6P7P

Structure of E. coli MS115-1 NucC, cAAA-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	131.592, 131.592, 252.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.796 - 1.665
R-factor	0.1429
Rwork	0.142
R-free	0.16210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p7o
RMSD bond length	0.008
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.850	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.093	0.601
Rmeas	0.098	0.636
Rpim	0.031	0.205
Number of reflections	97528	4200
<I/σ(I)>	20.5
Completeness [%]	99.4	87.4
Redundancy	10	9.3
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	17-24% PEG 3350, 0.1 M Na/K tartrate, and 25 mM Tris-HCl pH 8.0