6P7B
Crystal structure of Fowlpox virus resolvase and substrate Holliday junction DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 4 |
| Unit cell lengths | 143.510, 143.510, 56.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.803 - 3.317 |
| R-factor | 0.2133 |
| Rwork | 0.210 |
| R-free | 0.26770 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((dev_3366: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.803 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.054 | 0.947 |
| Number of reflections | 17192 | 6167 |
| <I/σ(I)> | 8.2 | 1.2 |
| Completeness [%] | 98.3 | 97.02 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 1.000 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M sodium cacodylate, pH6.6, 0.15M sodium chloride |
