6P7A

CRYSTAL STRUCTURE OF THE FOWLPOX VIRUS HOLLIDAY JUNCTION RESOLVASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 32
Unit cell lengths	82.620, 82.620, 68.756
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.845 - 3.081
R-factor	0.2716
Rwork	0.271
R-free	0.27900
Structure solution method	AB INITIO PHASING
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX ((dev_3512: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.845	3.200
High resolution limit [Å]	3.081	3.100
Rmerge	0.030	0.424
Number of reflections	9345	2480
<I/σ(I)>	13.1	2.2
Completeness [%]	96.3	97.72
Redundancy	2.6	2.6
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Trix/HCl, 10mM Cadmium hloride, 6% W/V PEG3350