6P72

Crystal Structure of the Cedar henipavirus Attachment G Glycoprotein global domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 65
Unit cell lengths	211.675, 211.675, 113.194
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.829 - 3.283
R-factor	0.2073
Rwork	0.206
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.421
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.108	0.045
Rmeas	0.105	0.048
Rpim	0.047	0.018	0.562
Total number of observations	320698
Number of reflections	43933	4503	4353
<I/σ(I)>	6.8
Completeness [%]	99.8	99.9	99.7
Redundancy	7.3	7.2	6.9
CC(1/2)		0.998	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES, pH 6.0, 15% PEG3350