6P6U
Crystal Structure of Monoclinic Rabbit Muscle Lactate Dehydrogenase with Four Tetramers as the Asymmetric Unit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-09-15 |
| Detector | SDMS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 160.572, 100.838, 223.102 |
| Unit cell angles | 90.00, 101.23, 90.00 |
Refinement procedure
| Resolution | 60.000 - 2.420 |
| R-factor | 0.18412 |
| Rwork | 0.181 |
| R-free | 0.21338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ldh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.281 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.280 |
| Rmeas | 0.280 | |
| Number of reflections | 3000 | |
| <I/σ(I)> | 7.4 | 3.3 |
| Completeness [%] | 88.0 | 22 |
| Redundancy | 6.5 | 3 |
| CC(1/2) | 0.970 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Sitting drop composed of equal amounts of 20% PEG 3350 in .10 M Tris buffer pH 7.0 and a 30 mg/ml stock protein solution containing 0.1 M Tris pH 7.0. Reservoir 20% PEG 3350 in water. |
