6P6P

Crystal structure of hemagglutinin from influenza virus A/Sichuan/2/1987 (H3N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-09-27
Detector	NOIR-1
Wavelength(s)	1.00003
Spacegroup name	C 1 2 1
Unit cell lengths	211.940, 149.940, 150.090
Unit cell angles	90.00, 135.01, 90.00

Refinement procedure

Resolution	47.470 - 2.310
R-factor	0.2299
Rwork	0.221
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yp5
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	2.393
High resolution limit [Å]	2.310	2.310
Rmeas	0.120	0.841
Rpim	0.085	0.594
Number of reflections	143375	13250
<I/σ(I)>	8.65	1.35
Completeness [%]	99.0	92.18
Redundancy	2	2
CC(1/2)	0.990	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M Hepes pH 7.0, 30% Jeffamine ED-2001