6P5L

Crystal Structure of Ubl123 with an EZH2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 42 21 2
Unit cell lengths	165.452, 165.452, 110.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.608 - 3.296
R-factor	0.2359
Rwork	0.231
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wpi
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.360
High resolution limit [Å]	3.296	3.300
Rmerge	0.060	0.940
Rpim	0.016	0.230
Number of reflections	23736	2303
<I/σ(I)>	49.6
Completeness [%]	99.3	99.4
Redundancy	28.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	167 mM NaCl, 20mM Tris, 5mM b-ME, 10mM betaine hydrochlorid