6P5D
Photoactive Yellow Protein PYP 30ps
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | EUROPEAN XFEL BEAMLINE SPB/SFX |
| Synchrotron site | European XFEL |
| Beamline | SPB/SFX |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2019-03-07 |
| Detector | AGIPD |
| Wavelength(s) | 1.33 |
| Spacegroup name | P 63 |
| Unit cell lengths | 66.900, 66.900, 40.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.810 - 1.600 |
| R-factor | 0.22275 |
| Rwork | 0.221 |
| R-free | 0.26651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.543 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 11.810 | 1.641 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 12318 | 811 |
| <I/σ(I)> | 13.98 | |
| Completeness [%] | 93.3 | |
| Redundancy | 287.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 293 | Sodium Malonate |
