6P57

Crystal Structure of the Beta Subunit of Luteinizing Hormone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X7B
Synchrotron site	NSLS
Beamline	X7B
Temperature [K]	298
Detector technology	CCD
Collection date	1998-06-12
Detector	MARRESEARCH
Wavelength(s)	1.1
Spacegroup name	F 2 2 2
Unit cell lengths	80.016, 80.016, 206.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.320 - 3.160
R-factor	0.21095
Rwork	0.207
R-free	0.28605
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hcn
RMSD bond length	0.010
RMSD bond angle	1.804
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	3.150
High resolution limit [Å]	3.000	3.000
Rmerge	0.150	0.640
Rmeas	0.160	0.680
Number of reflections	6465	480
<I/σ(I)>	9.1	3.3
Completeness [%]	98.4	98
Redundancy	9.35	9.6
CC(1/2)	0.890	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	Sitting drop with the drop composed of equal quantities of 20 mg/ml protein dissolved in water, and 20% PEG 3350 plus 1% beta octyl glucoside dissolved in 0.10 M MES buffer at pH 6.5. Room temperature crystallization.