6P57
Crystal Structure of the Beta Subunit of Luteinizing Hormone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X7B |
| Synchrotron site | NSLS |
| Beamline | X7B |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1998-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 80.016, 80.016, 206.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.320 - 3.160 |
| R-factor | 0.21095 |
| Rwork | 0.207 |
| R-free | 0.28605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hcn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.804 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.150 | 0.640 |
| Rmeas | 0.160 | 0.680 |
| Number of reflections | 6465 | 480 |
| <I/σ(I)> | 9.1 | 3.3 |
| Completeness [%] | 98.4 | 98 |
| Redundancy | 9.35 | 9.6 |
| CC(1/2) | 0.890 | 0.340 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Sitting drop with the drop composed of equal quantities of 20 mg/ml protein dissolved in water, and 20% PEG 3350 plus 1% beta octyl glucoside dissolved in 0.10 M MES buffer at pH 6.5. Room temperature crystallization. |
