6P4T
Salmonella typhi PltB Homopentamer T65I Mutant with Neu5Ac-alpha-2-3-Gal-beta-1-4-GlcNAc Glycans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-24 |
| Detector | RIGAKU HyPic-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.282, 97.714, 119.076 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.777 - 2.170 |
| R-factor | 0.1878 |
| Rwork | 0.186 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.955 |
| Data reduction software | CrysalisPro (40.43a) |
| Data scaling software | CrysalisPro (40.43a) |
| Phasing software | PHENIX (1.12-2829) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.779 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 48442 | 4846 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 98.5 | 96.6 |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Sodium Chloride, 0.1M Sodium Acetate pH 4.5, 1.26M Ammonium sulfate |
