6P4S
Salmonella typhi PltB Homopentamer T65I Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-22 |
| Detector | RIGAKU HyPic-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.068, 97.498, 118.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.643 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.869 |
| Data reduction software | CrysalisPro (40.43a) |
| Data scaling software | CrysalisPro (40.43a) |
| Phasing software | PHENIX (1.12-2829) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.645 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 48262 | 4828 |
| <I/σ(I)> | 14.15 | |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Sodium Chloride, 0.1M Sodium Acetate pH 4.5, 1.26M Ammonium sulfate |
