6P4R
Salmonella typhi PltB Homopentamer N29K Mutant with Neu5Ac-alpha-2-6-Gal-beta-1-4-GlcNAc glycans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-26 |
| Detector | RIGAKU HyPic-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.916, 96.827, 118.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.395 - 1.870 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.20550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.122 |
| Data reduction software | CrysalisPro (40.43a) |
| Data scaling software | CrysalisPro (40.43a) |
| Phasing software | PHENIX (1.12-2829) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.398 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 58793 | 5882 |
| <I/σ(I)> | 16.25 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG1500, Sodium actetate pH 4.4 |
