6P4Q
Salmonella typhi PltB Homopentamer N29K Mutant with Neu5Ac-alpha-2-3-Gal-beta-1-4-GlcNAc Glycans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-26 |
| Detector | RIGAKU HyPic-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.053, 96.894, 119.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.422 - 1.770 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | CrysalisPro (40.43a) |
| Data scaling software | CrysalisPro (40.43a) |
| Phasing software | PHENIX (1.12-2829) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.426 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 69206 | 6926 |
| <I/σ(I)> | 18.25 | |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG1500, Sodium actetate pH 4.4 |
